How a disordered linker in the Polycomb protein Polyhomeotic tunes phase separation and oligomerization.

ABSTRACT: Biomolecular condensates are increasingly appreciated for their function in organizing and regulating biochemical processes in cells, including chromatin function. Condensate formation and properties are encoded in protein sequence but the mechanisms linking sequence to macroscale properties are incompletely understood. Cross species comparisons can reveal mechanisms either because they identify conserved functions or because they point to important differences. Here we use in vitro reconstitution and molecular dynamics simulations to compare Drosophila and human sequences that regulate condensate formation driven by the sterile alpha motif (SAM) oligomerization domain in the Polyhomeotic (Ph) subunit of the chromatin regulatory complex PRC1. We discover evolutionarily diverg

SUBMITTER: Gemeinhardt TM 

PROVIDER: S-EPMC10634872 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature