RNA isoform expression landscape of the human dorsal root ganglion (DRG) generated from long read sequencing.

ABSTRACT: Splicing is a post-transcriptional RNA processing mechanism that enhances genomic complexity by creating multiple isoforms from the same gene. Diversity in splicing in the mammalian nervous system is associated with neuronal development, synaptic function and plasticity, and is also associated with diseases of the nervous system ranging from neurodegeneration to chronic pain. We aimed to characterize the isoforms expressed in the human peripheral nervous system, with the goal of creating a resource to identify novel isoforms of functionally relevant genes associated with somatosensation and nociception. We used long read sequencing (LRS) to document isoform expression in the human dorsal root ganglia (hDRG) from 3 organ donors. Isoforms were validated in silico by confirming express

SUBMITTER: Arendt-Tranholm A 

PROVIDER: S-EPMC10634934 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature