Deployment of a <i>Vibrio cholerae</i> ordered transposon mutant library in a quorum-competent genetic background.

ABSTRACT: Vibrio cholerae, the causative agent of cholera, has sparked seven pandemics in recent centuries, with the current one being the most prolonged. V. cholerae's pathogenesis hinges on its ability to switch between low and high cell density gene regulatory states, enabling transmission between host and the environment. Previously, a transposon mutant library for V. cholerae was created to support investigations aimed toward uncovering the genetic determinants of its pathogenesis. However, subsequent sequencing uncovered a mutation in the gene luxO of the parent strain, rendering mutants unable to exhibit high cell density behaviors. In this study, we used chitin-independent natural transformation to move transposon insertions from these low cell density mutants int

SUBMITTER: Grant NA 

PROVIDER: S-EPMC10634969 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature