An engineered thermally tolerant apo-cytochrome scaffold for metal-less incorporation of heme derivative.

ABSTRACT: Cytochrome c552 from Thermus thermophilus is one of the hot topics for creating smart biomaterials as it possesses remarkable stability, is tolerant to multiple mutations and has therefore been recently reported for a number of functionalizations upon substitution of the original prosthetic group with an artificial prosthetic group. However, all of the substitutions were driven by the coordination through the axial ligands followed by complete reconstitution with a metal-porphyrin complex. This limits the scope of the cytochrome c for incorporating a metal-less non-natural heme species that could improve the versatility of cytochrome c for a new generation of engineered cytochrome proteins for further enhancement in their functionalities such as biocatalysts. In this connection, a new vari

SUBMITTER: Ben Aoun S 

PROVIDER: S-EPMC10635480 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature