Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes.

ABSTRACT:

Objectives

This study aimed to investigate whether chromatography using an ExoPUA column, an affinity column for phospholipid membranes, could potentially serve as an efficient, rapid, scalable, and reproducible method for purifying small extracellular vesicles (sEVs).

Results

We used the ExoPUA column connected to a fast-performance liquid chromatography system. One-step chromatographic purification of sEVs from culture supernatant using the ExoPUA protocol resulted in an 82 ± 16-fold increase in purity with a yield of 38 ± 5% of sEVs. The purified sEVs contained CD9, CD63, TSG101, and miRNA (miR-21), but not the endoplasmic reticulum protein Calnexin. Transmission electron microscopy indicated that the purified sEVs were intact. The purification performance of the ExoPUA p

SUBMITTER: Masaki K 

PROVIDER: S-EPMC10635940 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature