Isolation, adaptation, and characterization of lumpy skin disease virus from cattle in Bangladesh.

ABSTRACT: The research aimed to isolate, adapt to cell culture, and characterize the lumpy skin disease virus (LSDV) from clinically infected cattle in Bangladesh. From September 2019 to June 2020, 37 skin nodules and skin swabs were aseptically collected from afflicted cattle in the outbreak regions of Jhenaidah and Kishoreganj in Bangladesh. The LSDV was isolated from embryonated specific pathogen-free (SPF) chicken eggs along the chorioallantoic membrane (CAM) route and the Vero cell line after several blind passages. The viral attachment protein was targeted for molecular detection using polymerase chain reactions (PCR). For phylogenetic analysis, PCR-positive products were partially sequenced. The virus was evident in the cell line, showed cytopathic effects after the 13 blind passage, and on t

SUBMITTER: Pervin S 

PROVIDER: S-EPMC10636076 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature