The integrative multi-omics approach identifies the novel competing endogenous RNA (ceRNA) network in colorectal cancer.

ABSTRACT: Circular RNAs (circRNA) are known to function as competing endogenous RNA (ceRNA) in various cancers by regulating microRNAs (miRNA). However, in colorectal cancer (CRC), the precise pathological role of circ000240/miRNA/mRNA remains indeterminate. The expression level of hsa_circ_000240 was evaluated using qRT-PCR in matching pairs of CRC tumor and adjacent normal tissue samples in our laboratory. Then, to determine whether hsa_circ_000240 acted as a ceRNA in CRC, the linked miRNAs and gene targets were retrieved. Topological analysis of candidate genes using a network approach identified the most critical hub genes and subnetworks related to CRC disease. Microarray and bulk RNA sequencing analyses were utilized to comprehensively evaluate the expression levels of both miRNA and mRNA in C

SUBMITTER: Mahmoodi Chalbatani G 

PROVIDER: S-EPMC10636147 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature