Orai1α and Orai1β support calcium entry and mammosphere formation in breast cancer stem cells.

ABSTRACT: Orai1 is the pore-forming subunit of the Ca²⁺-release activated Ca²⁺ channels that mediate store-operated Ca²⁺ entry (SOCE) in excitable and non-excitable cells. Two Orai1 forms have been identified in mammalian cells, the full-length variant Orai1α, and the short form Orai1β, lacking the N-terminal 63 amino acids. Stem cells were isolated from non-tumoral breast epithelial cells of the MCF10A cell line, and the most representative ER+ , HER2 or triple negative breast cancer cell lines MCF7, SKBR3 and MDA-MB-231, respectively. Orai and TRPC family members expression was detected by RT-PCR and Western blotting. Changes in cytosolic Ca²⁺ concentration were analyzed by confocal microscopy using Fluo 4 and the spheroid-forming ability and self-renewa

SUBMITTER: Jardin I 

PROVIDER: S-EPMC10636192 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature