AMP-dependent phosphite dehydrogenase, a phosphorylating enzyme in dissimilatory phosphite oxidation.

ABSTRACT: Oxidation of phosphite (HPO₃^2-) to phosphate (HPO₄^2-) releases electrons at a very low redox potential (E₀^'= -690 mV) which renders phosphite an excellent electron donor for microbial energy metabolism. To date, two pure cultures of strictly anaerobic bacteria have been isolated that run their energy metabolism on the basis of phosphite oxidation, the Gram-negative Desulfotignum phosphitoxidans (DSM 13687) and the Gram-positive Phosphitispora fastidiosa (DSM 112739). Here, we describe the key enzyme for dissimilatory phosphite oxidation in these bacteria. The enzyme catalyzed phosphite oxidation in the presence of adenosine monophosphate (AMP) to form adenosine diphosphate (ADP), with concomitant reduction of o

SUBMITTER: Mao Z 

PROVIDER: S-EPMC10636320 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature