A mechano- and heat-gated two-pore domain K⁺ channel controls excitability in adult zebrafish skeletal muscle.

ABSTRACT: TRAAK channels are mechano-gated two-pore-domain K⁺ channels. Up to now, activity of these channels has been reported in neurons but not in skeletal muscle, yet an archetype of tissue challenged by mechanical stress. Using patch clamp methods on isolated skeletal muscle fibers from adult zebrafish, we show here that single channels sharing properties of TRAAK channels, i.e., selective to K⁺ ions, of 56 pS unitary conductance in the presence of 5 mM external K⁺, activated by membrane stretch, heat, arachidonic acid, and internal alkaline pH, are present in enzymatically isolated fast skeletal muscle fibers from adult zebrafish. The kcnk4b transcript encoding for TRAAK channels was cloned and found, concomitantly with activity of mechano-gated K^+<

SUBMITTER: Idoux R 

PROVIDER: S-EPMC10636360 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature