Alterations in lung and gut microbiota reduce diversity in patients with nontuberculous mycobacterial pulmonary disease.

ABSTRACT:

Background/aims

Although the incidence of nontuberculous mycobacteria pulmonary disease (NTM-PD), a chronic infectious disease, is increasing, lung and gut microbiota dysbiosis in NTM patients has rarely been studied and was therefore the focus of this study.

Methods

We analyzed the microbiota diversity in sputum and stool samples from 10 healthy subjects and 10 patients with NTM-PD through sequencing of the V3 and V4 regions of the 16S rRNA gene. In NTM-PD patients, we comparatively evaluated the microbiota diversity according to the body mass index (BMI), with BMI ≤ 18.5 kg/m2 defined as "underweight" and BMI > 18.5 kg/m2 as "others."

Results

The sputum microbiota from NTM-PD patients tended to have lower index values of amplicon sequence variant richness, Shannon e

SUBMITTER: Choi JY 

PROVIDER: S-EPMC10636543 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature