Skeletal muscle regeneration failure in ischemic-damaged limbs is associated with pro-inflammatory macrophages and premature differentiation of satellite cells.

ABSTRACT:

Background

Chronic limb-threatening ischemia (CLTI), a severe manifestation of peripheral arterial disease (PAD), is associated with a 1-year limb amputation rate of approximately 15-20% and substantial mortality. A key feature of CLTI is the compromised regenerative ability of skeletal muscle; however, the mechanisms responsible for this impairment are not yet fully understood. In this study, we aim to delineate pathological changes at both the cellular and transcriptomic levels, as well as in cell-cell signaling pathways, associated with compromised muscle regeneration in limb ischemia in both human tissue samples and murine models of CLTI.

Methods

We performed single-cell transcriptome analysis of ischemic and non-ischemic muscle from the same CLTI patients and from a mur

SUBMITTER: Southerland KW 

PROVIDER: S-EPMC10636829 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature