Deep transcriptome profiling reveals limited conservation of A-to-I RNA editing in Xenopus.

ABSTRACT:

Background

Xenopus has served as a valuable model system for biomedical research over the past decades. Notably, ADAR was first detected in frog oocytes and embryos as an activity that unwinds RNA duplexes. However, the scope of A-to-I RNA editing by the ADAR enzymes in Xenopus remains underexplored.

Results

Here, we identify millions of editing events in Xenopus with high accuracy and systematically map the editome across developmental stages, adult organs, and species. We report diverse spatiotemporal patterns of editing with deamination activity highest in early embryogenesis before zygotic genome activation and in the ovary. Strikingly, editing events are poorly conserved across different Xenopus species. Even sites that are detected in both X. laevis and X. tropicalis s

SUBMITTER: Nguyen TA 

PROVIDER: S-EPMC10636886 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature