A novel transgenic mouse line with hippocampus-dominant and inducible expression of truncated human tau.

ABSTRACT:

Background

Intraneuronal accumulation of hyperphosphorylated tau is a defining hallmark of Alzheimer's disease (AD). However, mouse models imitating AD-exclusive neuronal tau pathologies are lacking.

Methods

We generated a new tet-on transgenic mouse model expressing truncated human tau N1-368 (termed hTau368), a tau fragment increased in the brains of AD patients and aged mouse brains. Doxycycline (dox) was administered in drinking water to induce hTau368 expression. Immunostaining and Western blotting were performed to measure the tau level. RNA sequencing was performed to evaluate gene expression, and several behavioral tests were conducted to evaluate mouse cognitive functions, emotion and locomotion.

Results

Dox treatment for 1-2 months at a young age induced ove

SUBMITTER: Gao Y 

PROVIDER: S-EPMC10637005 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature