In silico prospecting of the mtDNA of Macrobrachium amazonicum from transcriptome data.

ABSTRACT:

Background

Macrobrachium amazonicum is a freshwater prawn widely distributed in South America that is undergoing speciation, so the denomination "M. amazonicum complex" is used for it. The mitochondrial cytochrome c oxidase subunit I (COI) gene has been used to elucidate this speciation, but heteroplasmies and pseudogenes have been recorded, making separation difficult. Obtaining genes from cDNA (RNA) rather than genomic DNA is an effective tool to mitigate those two types of occurrences. The aim of this study was to assemble in silico the mitochondrial DNA (mtDNA) of the Amazonian coastal population of M. amazonicum inhabiting the state of Pará.

Results

Sequences were obtained from the prawn's transcriptome using the de novo approach. Six libraries of cDNA from the androgen

SUBMITTER: Marques-Neto JC 

PROVIDER: S-EPMC10637016 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature