Upregulated SPAG6 correlates with increased STAT1 and is associated with reduced sensitivity of interferon-α response in BCR::ABL1 negative myeloproliferative neoplasms.

ABSTRACT: Sperm-associated antigen 6 (SPAG6) has been identified as an oncogene or tumor suppressor in various types of human cancer. However, the role of SPAG6 in BCR::ABL1 negative myeloproliferative neoplasms (MPNs) remains unclear. Herein, we found that SPAG6 was upregulated at the mRNA level in primary MPN cells and MPN-derived leukemia cell lines. The SPAG6 protein was primarily located in the cytoplasm around the nucleus and positively correlated with β-tubulin expression. In vitro, forced expression of SPAG6 increased cell clone formation and promoted G₁ to S cell cycle progression. Downregulation of SPAG6 promoted apoptosis, reduced G₁ to S phase transition, and impaired cell proliferation and cytokine release accompanied by downregulated signal transducer and activato

SUBMITTER: Ding L 

PROVIDER: S-EPMC10637088 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature