Neuropathological Examination of Mice Chronically Exposed to Secondhand Smoke.

ABSTRACT:

Introduction

Around 21.6-35% of military personnel are smokers, while 12.26% of them have been regularly exposed to second-hand smoke (SHS). Second-hand smoke is considered an important risk factor for neurological diseases because it can induce oxidative stress, DNA damage, and disrupt DNA repair pathways.

Material and methods

The brain of air (sham) or SHS exposed mice was cryoperserved, sectioned, and placed on a glass slide before immunoprobing them with antibodies to observe for oxidative DNA damage (8-oxoG), oxidative DNA repair (8-oxoguanine DNA glycosylase 1, Ogg1; apurinic/apyrimidinic endonuclease, Ape1), and inflammatory (glial fibrillary acidic protein) proteins.

Results

Nissl staining of the prefrontal cortex (PFCTX) revealed the presence of dark, shrunke

SUBMITTER: Lopes LA 

PROVIDER: S-EPMC10637311 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature