Untapped Potential: Real-Time Measurements of Opioid Exocytosis at Single Cells.

ABSTRACT: The endogenous opioid system is commonly targeted in pain treatment, but the fundamental nature of neuropeptide release remains poorly understood due to a lack of methods for direct detection of specific opioid neuropeptides in situ. These peptides are concentrated in, and released from, large dense-core vesicles in chromaffin cells. Although catecholamine release from these neuroendocrine cells is well characterized, the direct quantification of opioid peptide exocytosis events has not previously been achieved. In this work, a planar carbon-fiber microelectrode served as a "postsynaptic" sensor for probing catecholamine and neuropeptide release dynamics via amperometric monitoring. A constant potential of 500 mV was employed for quantification of catecholamine release, and a higher potent

SUBMITTER: Denison JD 

PROVIDER: S-EPMC10637323 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature