Glycosylation of bacterial antigens changes epitope patterns.

ABSTRACT:

Introduction

Unlike glycosylation of proteins expressed in mammalian systems, bacterial glycosylation is often neglected in the development of recombinant vaccines.

Methods

Here, we compared the effects of glycosylation of YghJ, an Escherichia coli protein important for mucus attachment of bacteria causing in urinary tract infections (UTIs). A novel method based on statistical evaluation of phage display for the identification and comparison of epitopes and mimotopes of anti-YghJ antibodies in the sera was used. This is the first time that the effect of glycosylation of a recombinant bacterial antigen has been studied at the peptide epitope level.

Results

The study identifies differences in the immune response for (non)-glycosylated antigens in rabbits and pigs

SUBMITTER: Kern K 

PROVIDER: S-EPMC10637626 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature