PD-L1 regulates inflammatory programs of macrophages from human pluripotent stem cells.

ABSTRACT: Programmed death ligand 1 (PD-L1) serves as a pivotal immune checkpoint in both the innate and adaptive immune systems. PD-L1 is expressed in macrophages in response to IFNγ. We examined whether PD-L1 might regulate macrophage development. We established PD-L1 KO (CD274 ^-/- ) human pluripotent stem cells and differentiated them into macrophages and observed a 60% reduction in CD11B⁺CD45⁺ macrophages in CD274 ^-/- ; this was orthogonally verified, with the PD-L1 inhibitor BMS-1166 reducing macrophages to the same fold. Single-cell RNA sequencing further confirmed the down-regulation of the macrophage-defining transcription factors SPI1 and MAFB Furthermore, CD274 ^-/- macrophages red

SUBMITTER: Cao H 

PROVIDER: S-EPMC10638094 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature