Pancreatic GLUT2 protein expression and isolated islets insulin secretion decreased in high-fat fed rat dams.

ABSTRACT:

Purpose

Chronic consumption of high-fat foods during the reproductive period may endanger the dams' metabolic homeostasis and might adversely affect pregnancy outcome. In this regard the present study aimed to investigate the effect of long-term high-fat feeding on pancreatic glucose transporter-2 (GLUT2) protein expression and isolated islets glucose-stimulated insulin secretion in Wistar rat dams.

Materials and methods

Female rats were randomly divided into normal (N) and high-fat (HF; containing cow butter) diet groups and consumed their respective diets for 10 weeks (from prepregnancy to the end of lactation). After lactation, fasting plasma concentrations of glucose and insulin were measured to calculate HOMA-IR index, then intraperitoneal glucose tolerance test (IPGTT)

SUBMITTER: Karbaschi R 

PROVIDER: S-EPMC10638334 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature