Impact of volume and expression time in an AAV-delivered channelrhodopsin.

ABSTRACT: Optogenetics has revolutionised neuroscience research, but at the same time has brought a plethora of new variables to consider when designing an experiment with AAV-based targeted gene delivery. Some concerns have been raised regarding the impact of AAV injection volume and expression time in relation to longitudinal experimental designs. In this study, we investigated the efficiency of optically evoked post-synaptic responses in connection to two variables: the volume of the injected virus and the expression time of the virus. For this purpose, we expressed the blue-shifted ChR2, oChIEF, employing a widely used AAV vector delivery strategy. We found that the volume of the injected virus has a minimal impact on the efficiency of optically-evoked postsynaptic population responses. The expr

SUBMITTER: Ansarifar S 

PROVIDER: S-EPMC10638758 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature