Elucidating the role of nicotinamide N-methyltransferase-p53 axis in the progression of chronic kidney disease.

ABSTRACT:

Background

Chronic kidney disease (CKD) is a significant global health issue characterized by progressive loss of kidney function. Renal interstitial fibrosis (TIF) is a common feature of CKD, but current treatments are seldom effective in reversing TIF. Nicotinamide N-methyltransferase (NNMT) has been found to increase in kidneys with TIF, but its role in renal fibrosis is unclear.

Methods

Using mice with unilateral ureteral obstruction (UUO) and cultured renal interstitial fibroblast cells (NRK-49F) stimulated with transforming growth factor-β1 (TGF-β1), we investigated the function of NNMT in vivo and in vitro.

Results

We performed single-cell transcriptome sequencing (scRNA-seq) on the kidneys of mice and found that NNMT increased mainly in fibroblas

SUBMITTER: Zhen X 

PROVIDER: S-EPMC10638915 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature