Forster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features.

ABSTRACT: Förster resonance energy transfer (FRET) is an established fluorescence-based method used to successfully measure distances in and between biomolecules in vitro as well as within cells. In FRET, the efficiency of energy transfer, measured by changes in fluorescence intensity or lifetime, relates to the distance between two fluorescent molecules or labels. Determination of dynamics and conformational changes from the distances are just some examples of applications of this method to biological systems. Under certain conditions, this methodology can add to and enhance existing X-ray crystal structures by providing information regarding dynamics, flexibility, and adaptation to binding surfaces. We describe the use of FRET and associated distance determinations to elucidate structural properti

SUBMITTER: Northrop J 

PROVIDER: S-EPMC10639101 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature