Leptin favors imbalance of antigen-specific CD4⁺ T-cells associated with severity of cat allergy.

ABSTRACT:

Introduction

Obesity can complicate IgE-mediated allergic diseases. In the present study, we aimed to investigate the ability of obesity-related concentrations of leptin to modulate the in vitro effector and regulatory Fel d1-specific CD4⁺ T-cell subsets in patients allergic to cat, considered the third most common cause of respiratory allergy in humans.

Methods

For this study, plasma and peripheral blood mononuclear cells (PBMC) from 30 cat-allergic patients with mild, moderate and severe respiratory symptoms were obtained. The PBMC cultures were stimulated with Fel d1 antigen (10 µg/mL) in the presence or absence of obesity-related leptin dose (50 ηg/mL). After 6 days, the levels of cytokines and IgE in the supernatants were evaluated by multiplex and ELI

SUBMITTER: Vollmer C 

PROVIDER: S-EPMC10639137 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature