Recovery of microbial DNA by agar-containing solution from extremely low-biomass specimens including skin.

ABSTRACT: Recovering a sufficient amount of microbial DNA from extremely low-biomass specimens, such as human skin, to investigate the community structure of the microbiome remains challenging. We developed a sampling solution containing agar to increase the abundance of recovered microbial DNA. Quantitative PCR targeting the 16S rRNA gene revealed a significant increase in the amount of microbial DNA recovered from the developed sampling solution compared with conventional solutions from extremely low-biomass skin sites such as the volar forearm and antecubital fossa. In addition, we confirmed that the developed sampling solution reduces the contamination rate of probable non-skin microbes compared to the conventional solutions, indicating that the enhanced recovery of microbial DNA was accompanied

SUBMITTER: Kurokawa R 

PROVIDER: S-EPMC10640576 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature