Skeletal muscle cells opto-stimulation by intramembrane molecular transducers.

ABSTRACT: Optical stimulation and control of muscle cell contraction opens up a number of interesting applications in hybrid robotic and medicine. Here we show that recently designed molecular phototransducer can be used to stimulate C2C12 skeletal muscle cells, properly grown to exhibit collective behaviour. C2C12 is a skeletal muscle cell line that does not require animal sacrifice Furthermore, it is an ideal cell model for evaluating the phototransducer pacing ability due to its negligible spontaneous activity. We study the stimulation process and analyse the distribution of responses in multinuclear cells, in particular looking at the consistency between stimulus and contraction. Contractions are detected by using an imaging software for object recognition. We find a deterministic response to li

SUBMITTER: Venturino I 

PROVIDER: S-EPMC10640616 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature