Quantitative analysis of peroxisome tracks using a Hidden Markov Model.

ABSTRACT: Diffusion and mobility are essential for cellular functions, as molecules are usually distributed throughout the cell and have to meet to interact and perform their function. This also involves the cytosolic migration of cellular organelles. However, observing such diffusion and interaction dynamics is challenging due to the high spatial and temporal resolution required and the accurate analysis of the diffusional tracks. The latter is especially important when identifying anomalous diffusion events, such as directed motions, which are often rare. Here, we investigate the migration modes of peroxisome organelles in the cytosol of living cells. Peroxisomes predominantly migrate randomly, but occasionally they bind to the cell's microtubular network and perform directed migration, which is d

SUBMITTER: Svensson CM 

PROVIDER: S-EPMC10640649 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature