Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas.
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ABSTRACT:
SUBMITTER: Steiger L
PROVIDER: S-EPMC10641308 | biostudies-literature | 2023 Oct
REPOSITORIES: biostudies-literature
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Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas.
Steiger Laura L Baumann Zora Z Keller Lena L Böni-Schnetzler Marianne M Donath Marc Y MY Meier Daniel T DT
STAR protocols 20231027 4
Diabetes mellitus is a disease of the hormone-secreting endocrine pancreas. However, increasing evidence suggests that the exocrine pancreas is also involved in the pathogenesis of diabetes. In this protocol, we describe how to harvest both isolated islets and exocrine tissue from one mouse pancreas, followed by a detailed explanation of how to isolate and analyze immune cells using full-spectrum flow cytometry. ...[more]
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