Expression of genes and enzymes involved in ovarian steroidogenesis in relation to human follicular development.

ABSTRACT:

Introduction

Granulosa cells (GCs) and theca cells (TCs) play a pivotal role in human ovarian steroidogenesis, facilitating the conversion of cholesterol into sex steroids that regulate normal reproductive function. This study aims to explore the expression patterns of key enzymes that govern human ovarian steroidogenesis throughout follicle development, employing both genomic and immunological methodologies.

Methods

Follicles and GCs obtained from women undergoing ovarian tissue cryopreservation (OTC) and in vitro fertilisation treatment were utilized. Gene expression data were obtained from a Chinese study using RNA sequencing and from microarray data generated in our laboratory to comprehensively analyse gene expression profiles across distinct stages of follicular

SUBMITTER: Zheng M 

PROVIDER: S-EPMC10641382 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature