Screening of reliable reference genes for the normalization of RT-qPCR in chicken gastrointestinal tract.

ABSTRACT: The application of reverse transcription quantitative real-time PCR technology for the production of gene tissue expression profiles is a widely employed approach in molecular biology research. It is imperative to ascertain internal reference genes that exhibit stable expression across diverse tissues to ensure the precision of tissue gene expression profiles. While there have been studies documenting the most suitable reference genes for various tissues in chickens, there is a dearth of research on the identification of reference genes in the gastrointestinal (GI) tract of chickens. This study utilized 4 different algorithms (Delta CT, BestKeeper, NormFinder, and Genorm) to assess the stability of 19 internal reference genes in various GI tract tissues, including individual GI tract tissu

SUBMITTER: Chen J 

PROVIDER: S-EPMC10641542 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature