A novel sensitive hexaplex high-resolution melt assay for identification of five human Plasmodium species plus internal control.

ABSTRACT:

Background

The diagnosis of malaria infection in humans remains challenging, further complicated by mixed Plasmodium species infections, potentially altering disease severity and morbidity. To facilitate appropriate control measures and treatment, rapid, sensitive, and specific detection assays, including those for the second minor species, would be required. This study aimed to develop a multiplex high-resolution melting (hexaplex PCR-HRM) assay with seven distinct peaks corresponding to five Plasmodium species of the Plasmodium genus, and an internal control to limit false negatives providing quality assurance testing results.

Methods

Five species-specific primers for human malaria species were designed targeting on the Plasmodium 18 small subunit ribosomal RNA (18S rRNA)

SUBMITTER: Srisutham S 

PROVIDER: S-EPMC10641754 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature