High throughput method for detecting murine brain atrophy using a clinical 3T MRI.

ABSTRACT:

Background

There is a lack of understanding of the mechanisms by which the CNS is injured in multiple sclerosis (MS). Since Theiler's murine encephalomyelitis virus (TMEV) infection in SJL/J mice is an established model of progressive disability in MS, and CNS atrophy correlates with progressive disability in MS, we used in vivo MRI to quantify total ventricular volume in TMEV infection. We then sought to identify immunological and virological biomarkers that correlated with increased ventricular size.

Methods

Mice, both infected and control, were followed for 6 months. Cerebral ventricular volumes were determined by MRI, and disability was assessed by Rotarod. A range of immunological and virological measures was obtained using standard techniques.

Results

Disability

SUBMITTER: Linzey M 

PROVIDER: S-EPMC10641942 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature