Establishment and characterization of a non-gestational choriocarcinoma patient-derived xenograft model.

ABSTRACT:

Background

Non-gestational choriocarcinoma (NGC) is a rare subtype of malignant germ cell tumour and there is no consensus on its treatment. The lack of suitable preclinical models for NGC is a challenge in drug discovery research. Patient-derived xenograft (PDX) models recapitulate the tumour microenvironment of the original cancer tissue. Therefore, they have received considerable attention for studies on rare cancer. Here, we aimed to establish a PDX model from a patient with recurrent NGC.

Methods

Fresh NGC tumour tissue was immediately transplanted into a severely immune-deficient mouse (NOD.Cg-Prkdc^scid1l2rg^tm1Wjl/SzJ) and maintained for more than three in vivo passages. Subsequently, we evaluated the molecular characteristics of the PDX model usi

SUBMITTER: Oda Y 

PROVIDER: S-EPMC10642054 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature