Identification and analysis of differential miRNA-mRNA interactions in coronary heart disease: an experimental screening approach.

ABSTRACT:

Objective

This aim of this study is to screen the differential molecules of kidney deficiency and blood stasis (KDBS) syndrome in coronary heart disease by high-throughput sequencing. In addition, the study aims to verify the alterations in the expression levels of miR-4685-3p and its regulated downstream, namely, C1QC, C4, and C5, using quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA), and to determine whether the complement and coagulation cascade pathway is the specific pathogenic pathway.

Methods

Patients diagnosed with unstable angina pectoris with KDBS syndrome, patients with non-kidney deficiency blood stasis (NKDBS) syndrome, and a Normal group were recruited. The clinical symptoms of each group were further analyzed. Illumi

SUBMITTER: Wang J 

PROVIDER: S-EPMC10642340 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature