Quantifying Intracellular Nanoparticle Distributions with Three-Dimensional Super-Resolution Microscopy.

ABSTRACT: Super-resolution microscopy can transform our understanding of nanoparticle-cell interactions. Here, we established a super-resolution imaging technology to visualize nanoparticle distributions inside mammalian cells. The cells were exposed to metallic nanoparticles and then embedded within different swellable hydrogels to enable quantitative three-dimensional (3D) imaging approaching electron-microscopy-like resolution using a standard light microscope. By exploiting the nanoparticles' light scattering properties, we demonstrated quantitative label-free imaging of intracellular nanoparticles with ultrastructural context. We confirmed the compatibility of two expansion microscopy protocols, protein retention and pan-expansion microscopy, with nanoparticle uptake studies. We validated relat

SUBMITTER: Sheth V 

PROVIDER: S-EPMC10643044 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature