Histamine H₁- and H₄-receptor expression in human colon-derived cell lines.

ABSTRACT: In previous studies, we demonstrated the involvement of H₄R in inflammatory bowel disease (IBD) and IBD-associated colon cancer in mice and could ascribe H₄R-mediated histamine function to colon epithelial cells. The transferability of obtained data to humans is however lacking. Functional expression of H₄R on colon epithelial cells is a prerequisite to pursue the hypothesis of involvement of H₄R in carcinogenesis. Thus, we here compared the expression of histamine receptor subtypes in a series of cell lines. Out of these, three colon-derived cell lines displaying different combinations of H₁R and H₄R expression were submitted to functional analyses. Human hematopoietic HMC-1, HL-60, and U937, lung-derived A549 and Calu-3, a

SUBMITTER: Schrammel JC 

PROVIDER: S-EPMC10643376 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature