Multifocal optical projection microscopy enables label-free 3D measurement of cardiomyocyte cluster contractility.

ABSTRACT: Human induced pluripotent stem cell (hiPSC)-derived cardiomyocyte (CM) models have become an attractive tool for in vitro cardiac disease modeling and drug studies. These models are moving towards more complex three-dimensional microphysiological organ-on-chip systems. Label-free imaging-based techniques capable of quantifying contractility in 3D are needed, as traditional two-dimensional methods are ill-suited for 3D applications. Here, we developed multifocal (MF) optical projection microscopy (OPM) by integrating an electrically tunable lens to our in-house built optical projection tomography setup for extended depth of field brightfield imaging in CM clusters. We quantified cluster biomechanics by implementing our previously developed optical flow-based CM video analysis for MF-OPM. To

SUBMITTER: Belay B 

PROVIDER: S-EPMC10643565 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature