Whole genome sequencing reveals insights into hepatitis E virus genome diversity, and virus compartmentalization in chronic hepatitis E.

ABSTRACT:

Background

Hepatitis E virus (HEV) is a leading cause of acute hepatitis and can cause chronic infections in immunocompromised patients. Although HEV infections can be treated with ribavirin, antiviral efficacy is hampered by resistance mutations, normally detected by virus sequencing.

Objectives

High-throughput sequencing (HTS) allows for cost-effective complete viral genome sequencing. This enables the discovery and delineation of new subtypes, and revised the recognition of quasispecies and putative resistance mutations. However, HTS is challenged by factors including low viral load, sample degradation, high host background, and high viral diversity.

Study design

We apply complete genome sequencing strategies for HEV, including a targeted enrichment approach. These

SUBMITTER: Melchert J 

PROVIDER: S-EPMC10643812 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature