Human CD79b⁺ neutrophils in the blood are associated with early-stage melanoma.

ABSTRACT:

Purpose

Due to their abundance in the blood, low RNA content, and short lifespan, neutrophils have been classically considered to be one homogenous pool. However, recent work has found that mature neutrophils and neutrophil progenitors are composed of unique subsets exhibiting context-dependent functions. In this study, we ask if neutrophil heterogeneity is associated with melanoma incidence and/or disease stage.

Experimental design

Using mass cytometry, we profiled melanoma patient blood for unique cell surface markers among neutrophils. Markers were tested for their predictiveness using flow cytometry data and random forest machine learning.

Results

We identified CD79b⁺ neutrophils (CD3^-CD56^-CD19^-Siglec8^-CD203c<

SUBMITTER: Meyer MA 

PROVIDER: S-EPMC10643866 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature