Ex vivo and in vitro Monocyte Responses Do Not Reflect in vivo Immune Responses and Tolerance.

ABSTRACT: Cytokine production by ex vivo (EV)-stimulated leukocytes is commonly used to gauge immune function and frequently proposed to guide immunomodulatory therapy. However, whether EV cytokine production capacity accurately reflects the in vivo (IV) immune status is largely unknown. We investigated relationships between EV monocyte cytokine responses and IV cytokine responses in a large cohort of healthy volunteers using a highly standardized IV model of short-lived LPS-induced systemic inflammation, which captures hallmarks of both hyperinflammation and immunological tolerance. Therefore, 110 healthy volunteers were intravenously challenged with 1 ng/kg LPS twice: on day 0 to determine the extent of the IV (hyper)inflammatory response and on day 7 to determine the degree of IV endotoxin tolera

SUBMITTER: Jansen A 

PROVIDER: S-EPMC10643897 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature