Autofluorescence imaging of endogenous metabolic cofactors in response to cytokine stimulation of classically activated macrophages.

ABSTRACT:

Background

Macrophages are one of the most prevalent subsets of immune cells within the tumor microenvironment and perform a range of functions depending on the cytokines and chemokines released by surrounding cells and tissues. Recent research has revealed that macrophages can exhibit a spectrum of phenotypes, making them highly plastic due to their ability to alter their physiology in response to environmental cues. Recent advances in examining heterogeneous macrophage populations include optical metabolic imaging, such as fluorescence lifetime imaging (FLIM), and multiphoton microscopy. However, the method of detection for these systems is reliant upon the coenzymes NAD(P)H and FAD, which can be affected by factors other than cytoplasmic metabolic changes. In this study, we seek

SUBMITTER: Bess SN 

PROVIDER: S-EPMC10644562 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature