Alterations in urinary microbiota composition in urolithiasis patients: insights from 16S rRNA gene sequencing.

ABSTRACT:

Objectives

To investigate the urinary microbiota composition in urolithiasis patients compared to healthy controls and to identify potential microbial markers and their association with clinical parameters.

Methods

A total of 66 samples, comprising 45 from urolithiasis patients and 21 from healthy controls, were analyzed. 16S rRNA gene sequencing was employed to determine the microbiota composition. Various statistical and bioinformatics tools, including ANOVA, PCoA, and LEfSe, were utilized to analyze the sequencing data and identify significant differences in microbial abundance.

Results

No significant demographic differences were observed between the two groups. Post-quality control, clean tags ranged from 60,979 to 68,736. Significant differences in α-diversity we

SUBMITTER: Liu H 

PROVIDER: S-EPMC10644862 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature