Tumor-on-a-chip platform to investigate progression and drug sensitivity in cell lines and patient-derived organoids.

ABSTRACT: Most cancer treatment strategies target cell proliferation, angiogenesis, migration, and intravasation of tumor cells in an attempt to limit tumor growth and metastasis. An in vitro platform to assess tumor progression and drug sensitivity could provide avenues to enhance our understanding of tumor metastasis as well as precision medicine. We present a microfluidic platform that mimics biological mass transport near the arterial end of a capillary in the tumor microenvironment. A central feature is a quiescent perfused 3D microvascular network created prior to loading tumor cells or patient-derived tumor organoids in an adjacent compartment. The physiological delivery of nutrients and/or drugs to the tumor then occurs through the vascular network. We demonstrate the culture, growth, and tr

SUBMITTER: Shirure VS 

PROVIDER: S-EPMC10644986 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature