Mechanism of substrate binding and transport in BASS transporters.

ABSTRACT: The bile acid sodium symporter (BASS) family transports a wide array of molecules across membranes, including bile acids in humans, and small metabolites in plants. These transporters, many of which are sodium-coupled, have been shown to use an elevator mechanism of transport, but exactly how substrate binding is coupled to sodium ion binding and transport is not clear. Here, we solve the crystal structure at 2.3 Å of a transporter from Neisseria meningitidis (ASBT_NM) in complex with pantoate, a potential substrate of ASBT_NM. The BASS family is characterised by two helices that cross-over in the centre of the protein in an arrangement that is intricately held together by two sodium ions. We observe that the pantoate binds, specifically, between the N-termini of

SUBMITTER: Becker P 

PROVIDER: S-EPMC10645422 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature