Modeling and analysis of voltage gating of gap junction channels at a single-channel level.

ABSTRACT: The advancement of single-channel-level recording via the patch-clamp technique has provided a powerful means of assessing the detailed behaviors of various types of ion channels in native and exogenously expressed cellular environments. However, such recordings of gap junction (GJ) channels are hampered by unique challenges that are related to their unusual intercellular configuration and natural clustering into densely packed plaques. Thus, the methods for reliable cross-correlation of data recorded at macroscopic and single-channel levels are lacking in studies of GJs. To address this issue, we combined our previously published four-state model (4SM) of GJ channel gating by voltage with maximum likelihood estimation (MLE)-based analyses of electrophysiological recordings of GJ channel c

SUBMITTER: Snipas M 

PROVIDER: S-EPMC10645554 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature