Propagation of PrP^Sc in mice reveals impact of aggregate composition on prion disease pathogenesis.

ABSTRACT: Infectious prions consist of PrP^Sc, a misfolded, aggregation-prone isoform of the host's prion protein. PrP^Sc assemblies encode distinct biochemical and biological properties. They harbor a specific profile of PrP^Sc species, from small oligomers to fibrils in different ratios, where the highest infectivity aligns with oligomeric particles. To investigate the impact of PrP^Sc aggregate complexity on prion propagation, biochemical properties, and disease pathogenesis, we fractionated elk prions by sedimentation velocity centrifugation, followed by sub-passages of individual fractions in cervidized mice. Upon first passage, different fractions generated PrP^Sc with distinct biochemical, biophysical, and neuropathological profiles. Notably,

SUBMITTER: Chang SC 

PROVIDER: S-EPMC10645910 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature