Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment.

ABSTRACT: A eukaryotic cell is a microscopic world within which efficient material transport is essential. Yet, how a cell manages to deliver cellular cargos efficiently in a crowded environment remains poorly understood. Here, we used interferometric scattering microscopy to track unlabeled cargos in directional motion in a massively parallel fashion. Our label-free, cargo-tracing method revealed not only the dynamics of cargo transportation but also the fine architecture of the actively used cytoskeletal highways and the long-term evolution of the associated traffic at sub-diffraction resolution. Cargos frequently run into a blocked road or experience a traffic jam. Still, they have effective strategies to circumvent those problems: opting for an alternative mode of transport and moving together i

SUBMITTER: Park JS 

PROVIDER: S-EPMC10645962 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature