Mechanistic insights into glycoside 3-oxidases involved in C-glycoside metabolism in soil microorganisms.

ABSTRACT: C-glycosides are natural products with important biological activities but are recalcitrant to degradation. Glycoside 3-oxidases (G3Oxs) are recently identified bacterial flavo-oxidases from the glucose-methanol-coline (GMC) superfamily that catalyze the oxidation of C-glycosides with the concomitant reduction of O₂ to H₂O₂. This oxidation is followed by C-C acid/base-assisted bond cleavage in two-step C-deglycosylation pathways. Soil and gut microorganisms have different oxidative enzymes, but the details of their catalytic mechanisms are largely unknown. Here, we report that PsG3Ox oxidizes at 50,000-fold higher specificity (k_cat/K_m) the glucose moiety of mangiferin to 3-keto-mangiferin than free D-glucose to 2-keto-glucose. Analysis

SUBMITTER: Taborda A 

PROVIDER: S-EPMC10646112 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature