Experimental procedures for flow cytometry of wild-type mouse brain: a systematic review.

ABSTRACT:

Objective

The aim of this study was to systematically review the neuroimmunology literature to determine the average immune cell counts reported by flow cytometry in wild-type (WT) homogenized mouse brains.

Background

Mouse models of gene dysfunction are widely used to study age-associated neurodegenerative disorders, including Alzheimer's disease and Parkinson's disease. The importance of the neuroimmune system in these multifactorial disorders has become increasingly evident, and methods to quantify resident and infiltrating immune cells in the brain, including flow cytometry, are necessary. However, there appears to be no consensus on the best approach to perform flow cytometry or quantify/report immune cell counts. The development of more standardized methods would accel

SUBMITTER: Sharp RC 

PROVIDER: S-EPMC10646240 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature